copt2ctr1抗体价格-博天堂备用
价格： ￥1880
品牌：晶风生物
货号：tf10622r
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抗体名：
ctr1抗体
抗体英文名：
copt2
靶点：
细胞浆 细胞膜 分泌型蛋白
浓度：
1mg/ml
应用范围：
elisa=1:500-1000,ihc-p=1:100-500,ihc-f=1:100-500,if=1:100-500,icc=1:100-500,
宿主：
rabbit
适应物种：
human,mouse,rat,dog,pig,cow,rabbit,sheep,guinea pig,
保质期：
一年
抗原来源：
rabbit
目录编号：
tf10622r
级别：
i级
库存：
10
供应商：
晶风生物
标记物：
fitc/alexa/cy357/bio/hrp
克隆性：
polyclonal
保存条件：
-20
形态：
liquid
亚型：
igg
免疫原：
klh conjugated synthetic peptide derived
规格：
50ul/100ul/200ul
产品名称：copt2ctr1抗体
产品规格：100ul/200ul(部分有50ul，如需更大包装或其他具体规格，请咨询客服)
研究领域：肿瘤 细胞生物 免疫学等
抗体来源：rabbit
克隆类型：polyclonal
交叉反应：human,mouse,rat,dog,pig,cow,rabbit,sheep,
产品应用：wb=1:500-2000 elisa=1:5000-10000 ihc-p=1:100-500 ihc-f=1:100-500 flow-cyt=1μg/test if=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状：liquid
浓 度：1mg/ml
免 疫 原：klh conjugated synthetic peptide derived
亚 型：igg
纯化方法：affinity purified by protein a
储 存 液：0.01m tbs(ph7.4) with 1% bsa, 0.03% proclin300 and 50% glycerol.
保存条件：shipped at 4℃. store at -20 °c for one year. avoid repeated freeze/thaw cycles.
copt2ctr1抗体相关抗体示例（非本抗体，如需本抗体，请联系客服索要说明书）：
sample:
liver (mouse) lysate at 40 ug
spleen (mouse) lysate at 40 ug
nih/3t3 (mouse) celllysate at 30 ug
raw246.7 (mouse) celllysate at 30 ug
primary: anti- il12 at 1/300 dilution
secondary: irdye800cw goat anti-rabbit igg at 1/20000 dilution
predicted band size: 22 kd
observed band size: 35/36 kd
paraffin embedded (mouse brain); antigen retrieval by boiling in sodium citrate buffer (ph6.0) for 15min; block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; blocking buffer (normal goat serum) at 37°c for 30min; antibody incubation with (il12) polyclonal antibody, unconjugated at 1:400 overnight at 4°c, followed by operating according to sp kit(rabbit) instructions and dab staining.
paraffin embedded (rat brain tissue); antigen retrieval by boiling in sodium citrate buffer (ph6.0) for 15min; block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; blocking buffer (normal goat serum) at 37°c for 30min; antibody incubation with (il12) polyclonal antibody, unconjugated at 1:400 overnight at 4°c, followed by operating according to sp kit(rabbit) instructionsand dab staining.
copt2ctr1抗体
blank control (blue line): mouse spleen (blue).
primary antibody (green line): rabbit anti- il12 antibody
dilution: 1μg /10^6 cells;
isotype control antibody (orange line): rabbit igg .
secondary antibody (white blue line): goat anti-rabbit igg-fitc
dilution: 1μg /test.
protocol
the cells were fixed with 70% ice-cold methanol overnight at 4℃. cells stained with primary antibody for 30 min at room temperature. the cells were then incubated in 1 x pbs/2%bsa/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. the secondary antibody used for 40 min at room temperature. acquisition of 20,000 events was performed.
tissue/cell: rat colitis tissue; paraffin-embedded;
antigen retrieval: citrate buffer ( 0.01m, ph 6.0 ), boiling bathing for 15min; block endogenous peroxidase by 3% hydrogen peroxide for 30min; blocking buffer (normal goat serum,at 37℃ for 20 min;
incubation: anti-il-12 polyclonal antibody, unconjugated 1:200, overnight at 4°c, followed by conjugation to the secondary antibody(sp-0023) and dab staining
欢迎新老客户咨询订购：copt2ctr1抗体
产品规格：100ul/200ul(部分有50ul，如需更大包装或其他具体规格，请咨询客服)
研究领域：肿瘤 细胞生物 免疫学等
抗体来源：rabbit
克隆类型：polyclonal
交叉反应：human,mouse,rat,dog,pig,cow,rabbit,sheep,
产品应用：wb=1:500-2000 elisa=1:5000-10000 ihc-p=1:100-500 ihc-f=1:100-500 flow-cyt=1μg/test if=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状：liquid
浓 度：1mg/ml
免 疫 原：klh conjugated synthetic peptide derived
亚 型：igg
纯化方法：affinity purified by protein a
储 存 液：0.01m tbs(ph7.4) with 1% bsa, 0.03% proclin300 and 50% glycerol.
保存条件：shipped at 4℃. store at -20 °c for one year. avoid repeated freeze/thaw cycles.
copt2ctr1抗体相关抗体示例（非本抗体，如需本抗体，请联系客服索要说明书）：
sample:
liver (mouse) lysate at 40 ug
spleen (mouse) lysate at 40 ug
nih/3t3 (mouse) celllysate at 30 ug
raw246.7 (mouse) celllysate at 30 ug
primary: anti- il12 at 1/300 dilution
secondary: irdye800cw goat anti-rabbit igg at 1/20000 dilution
predicted band size: 22 kd
observed band size: 35/36 kd
paraffin embedded (mouse brain); antigen retrieval by boiling in sodium citrate buffer (ph6.0) for 15min; block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; blocking buffer (normal goat serum) at 37°c for 30min; antibody incubation with (il12) polyclonal antibody, unconjugated at 1:400 overnight at 4°c, followed by operating according to sp kit(rabbit) instructions and dab staining.
paraffin embedded (rat brain tissue); antigen retrieval by boiling in sodium citrate buffer (ph6.0) for 15min; block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; blocking buffer (normal goat serum) at 37°c for 30min; antibody incubation with (il12) polyclonal antibody, unconjugated at 1:400 overnight at 4°c, followed by operating according to sp kit(rabbit) instructionsand dab staining.
copt2ctr1抗体
blank control (blue line): mouse spleen (blue).
primary antibody (green line): rabbit anti- il12 antibody
dilution: 1μg /10^6 cells;
isotype control antibody (orange line): rabbit igg .
secondary antibody (white blue line): goat anti-rabbit igg-fitc
dilution: 1μg /test.
protocol
the cells were fixed with 70% ice-cold methanol overnight at 4℃. cells stained with primary antibody for 30 min at room temperature. the cells were then incubated in 1 x pbs/2%bsa/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. the secondary antibody used for 40 min at room temperature. acquisition of 20,000 events was performed.
tissue/cell: rat colitis tissue; paraffin-embedded;
antigen retrieval: citrate buffer ( 0.01m, ph 6.0 ), boiling bathing for 15min; block endogenous peroxidase by 3% hydrogen peroxide for 30min; blocking buffer (normal goat serum,at 37℃ for 20 min;
incubation: anti-il-12 polyclonal antibody, unconjugated 1:200, overnight at 4°c, followed by conjugation to the secondary antibody(sp-0023) and dab staining
欢迎新老客户咨询订购：copt2ctr1抗体
联系人：骆飞
地址：上海
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