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anti-slc31a1/ctr1 antibody(a03447-1-150ul)

anti-博天堂备用

价格: ¥2780

品牌:boster 品牌认证

货号:a03447-1-150ul

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库存:
大量
供应商:
广州威佳科技有限公司
规格:
150ul

产品概况

货号 a03447-1
产品名称 anti-ctr1/slc31a1 antibody
基因名 slc31a1
抗体来源 rabbit
克隆 polyclonal
抗体亚型 rabbit igg
分子量 26-34kd
免疫原 a synthetic peptide corresponding to a sequence of human slc31a1/ctr1 (ngtilmethktvgqqmlsfphllqtvlhiiq).
内容 500 ug/ml antibody with pbs ,0.02% nan3 , 1 mg bsa and 50% glycerol.
纯化方式 immunogen affinity purified.
浓度 500 ug/ml
产品形态 liquid
保存条件 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. avoid repeated freezing and thawing.
背景资料 high affinity copper uptake protein 1 (ctr1) is a protein that in humans is encoded by the slc31a1 gene. this gene is maped to 9q32. the protein encoded by this gene is a high-affinity copper transporter found in the cell membrane. the encoded protein functions as a homotrimer to effect the uptake of dietary copper.
研究类别 1. eisses, j. f., kaplan, j. h. molecular characterization of hctr1, the human copper uptake protein. j. biol. chem. 277: 29162-29171, 2002. 2. klomp, a. e. m., tops, b. b. j., van den berg, i. e. t., berger, r., klomp, l. w. j. biochemical characterization and subcellular localization of human copper transporter 1 (hctr1). biochem. j. 364: 497-505, 2002.3. kuo, y.-m., zhou, b., cosco, d., gitschier, j. the copper transporter ctr1 provides an essential function in mammalian embryonic development. proc. nat. acad. sci. 98: 6836-6841, 2001.
uniprot id slc31a1:
推荐配套的二抗和检测试剂 boster recommends enhanced chemiluminescent kit with anti-rabbit igg (ek1002) for western blot, and hrp conjugated anti-rabbit igg super vision assay kit (sv0002-1) for ihc(p). *blocking peptide 可以联系博天堂在线开户网站购买。

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[list_product_images]figure 1. western blot analysis of slc31a1/ctr1 using anti-slc31a1/ctr1 antibody (a03447-1).electrophoresis was performed on a 5-20% sds-page gel at 70v (stacking gel) / 90v (resolving gel) for 2-3 hours. the sample well of each lane was loaded with 50ug of sample under reducing conditions.lane 1: human hepg2 whole cell lysates,lane 2: human panc-1 whole cell lysates.after electrophoresis, proteins were transferred to a nitrocellulose membrane at 150ma for 50-90 minutes. blocked the membrane with 5% non-fat milk/ tbs for 1.5 hour at rt. the membrane was incubated with rabbit anti-slc31a1/ctr1 antigen affinity purified polyclonal antibody (catalog # a03447-1) at 0.5 μg/ml overnight at 4°c, then washed with tbs-0.1%tween 3 times with 5 minutes each and probed with a goat anti-rabbit igg-hrp secondary antibody at a dilution of 1:10000 for 1.5 hour at rt. the signal is developed using an enhanced chemiluminescent detection (ecl) kit (catalog # ek1002) with tanon 5200 system. a specific band was detected for slc31a1/ctr1 at approximately 26kd. the expected band size for slc31a1/ctr1 is at 21kd.[/list_product_images]

联系人:张先生

地址:广州

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